International Journal of Current Research and Academic Review

ISSN: 2347-3215 Volume 1 Number 3 (2013) pp. 89-95

Phenotypic detection of ESBL and MBL in clinical isolates of Enterobacteriaceae

Mita D.Wadekar*, K.Anuradha and D.Venkatesha

Department of Microbiology, Mysore Medical College and Research, Institute, Mysore, Karnataka, India.

A B S T R A C T

Antimicrobial resistance is a growing threat worldwide. Increasing resistance to third generation cephalosporins has become a cause for concern among Enterobacteriaceae. The prevalence of extended spectrum -lactamases (ESBLs) and metallobetalactamases (MBL) among members of Enterobacteriaceae constitutes a serious threat to current -lactam therapy leading to treatment failure. 100 Enterobacteriaceae isolated from various clinical samples were included in the study. ESBL was detected by phenotypic confirmatory disc diffusion test (PCDDT) using ceftazidime alone and in combination with clavulanic acid. MBL detection was done by Imipenem EDTA combined disc diffusion test. Out of 100 Enterobacteriaceae isolates, 43(43%) were ESBL producers and 18(18%) MBL producers. None of the isolates showed the coexistence of ESBL and MBL in the same isolate. ESBL and MBL production was observed in E.coli, Klebsiella spp., Enterobacter spp. and Citrobacter spp. isolated from various clinical samples. The study underlines problem of ESBL and MBL mediated resistance, which has created a therapeutic challenge for the clinicians and microbiologists. Simple disk method can be routinely employed to detect these common resistance mechanisms which will reduce the mortality and also spread of such resistant strains.

Keywords

ESBL; MBL; Enterobacteriaceae.