Abstract            Volume:10  Issue-6  Year-2022         Original Research Articles

Online ISSN : 2347 - 3215 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcret@gmail.com

Nanotechnology is an emerging field of science. It has increased applications in diverse area for the development of new materials at nanoscale levels. Synthesis of nanoparticles using biological methods is referred as greener synthesis of nanoparticles. Seed extracts of papaya (Carica papaya) and Mullatha (Annona muricata) are used for the synthesis of silver, copper, and zinc nanoparticles. These plants have medicinal as well as antibacterial activity. Nanoparticles prepared from these seed extracts have antibacterial activity. Synthesized nanoparticles were characterized by UV-VIS Spectrophotometry. Silver nanoparticles shows maximum peak at 385 nm. Copper nanoparticles shows maximum peak at 680 nm. Zinc nanoparticles shows maximum peak at 350 nm. Synthesized silver, copper and zinc nanoparticles shows antibacterial activity against Salmonella species, Pseudomonas species, Staphylococcus species, E. coli and Klebsiella species. Antimicrobial assay was performed by agar well diffusion method using Muller Hinton agar media. when antibacterial activity of silver, copper and zinc nanoparticles from 3 different concentrations were observed, nanoparticles have 60 µl concentration shows maximum activity against these microbes. Silver nanoparticles shows greater antibacterial activity compared to silver nitrate and seed extract. Copper nanoparticles shows greater antibacterial activity compared to copper Sulphate and seed extract. Zinc nanoparticles shows greater antibacterial activity compared to zinc Sulphate and seed extract. Maximum zone of inhibition was at 60 µl for all the bacterial cultures. This green synthesis method is alternative to chemical method, since it is cheap, pollutant free and eco-friendly.

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