Abstract            Volume:10  Issue-3  Year-2022         Original Research Articles

Online ISSN : 2347 - 3215 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcret@gmail.com

In the face of predicted increases in the world population to around 10 billion by 2050 and the challenges faced by agriculture as a result of climate change, providing adequate food and fiber for humanity is a pressing issue requiring urgent attention. Therefore, the efforts to develop stress tolerant plants are of immense importance to increase crop productivity. In recent years, tissue culture based in vitro selection has emerged as a feasible and cost-effective tool for developing stress-tolerant plants. Plants tolerant to biotic stresses can be acquired by applying the selecting agents such as pathogen culture filtrate, phytotoxin or pathogen itself (for disease resistance) in the culture media. Only the explants capable of sustaining such environments survive in the long run and are selected. In vitro selection is based on the induction of genetic variation among cells, tissues and/or organs in cultured and regenerated plants. The selection of somaclonal variations appearing in the regenerated plants may be genetically stable and useful in crop improvement. This review focuses on the progress made towards the development of biotic stress-tolerant lines through tissue culture based in vitro selection.

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