Abstract            Volume:9  Issue-4  Year-2021         Original Research Articles

Online ISSN : 2347 - 3215 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcret@gmail.com

This study was carried out with the objective of evaluating the potential Resistance of released coffee Varieties for Bacterial Blight of Coffee (BBC) under laboratory conditions of the causative agent (Pseudomonas syringae pv. garcae). Bacteria isolates from four coffee growing areas in Sidama and Gedeo zones were characterized based on, biochemical and pathogen city characteristics to distinguish the pathogenic isolates of P. syringae from other phylloplane epiphytic bacteria. Isolates of bacteria from diseased coffee plants were categorized in three groups. Isolates that were pathogenic to coffee and produced typical symptoms of BBC fell under group 1 and were assumed to be of P. syringae pv. garcae. Other isolates that fell under groups 2 and 3 were considered to be saprophytic epiphytes. Nine commercially available and one promising coffee varieties were tested at Jimma plant pathological laboratory pathogenic bacteria isolates collected from the two zones. Inoculation was done with a drop of 9 approximately 10µl of the isolates suspension (10 CFU/ml) using injection method on 4 months old 0 seedlings from potting. Incubation temperature was set at 19-23 C and relative humidity maintained at 78%. The experiment was carried out in a Complete Randomized Design. Disease symptoms were scored using a scale of 1 to 5 and the data subjected to analysis of variance and effect declared significant at 5% level. The results revealed significant difference among the coffee genotypes of south and southwest with G-14/13 being the most resistant. Odicha coffee variety had scored the highest incidence bacterial blight while the promising (G-14/13) was showed least incidence of bacterial blight. It needs further Mass screening research because G-14/2013 from Wollega collection is an indication of the presence of resistant coffee genotypes at different coffee producing areas of Ethiopia.

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