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Abstract                 Volume:5  Issue-8  Year-2017          Original Research Articles

Online ISSN : 2347 - 3215
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcret@gmail.com

In Vitro Propagation of Bambusa vulgaris through Branch Node
Hardik Gajjar1, Akhil Raval1, Hemangini Raval2, Harikrushna Patel2 and Suthar Ramchandra2*
1Department of Microbiology, Shree M.M. Patel Institute of Science & Research, Kadi. KSV University, Gandhinagar. Gujarat, India
2Department of Biotechnology, P. S. Science & H. D. Patel Arts College, Kadi-382517, HNGU University, Patan, Gujarat, India
*Corresponding author

Low cost, efficient and reproducible protocol has been established through the technique of Plant tissue culture forced branch node for the propagation of an important bamboo species namely Bambusa vulgaris. The propagation of B. vulgaris through branch node culture was studied. Single node segments were tested for bud-break and shoot growth on basic Murashige and Skoog (1962) medium (MS) supplemented with different combinations and concentrations of growth regulators. Results suggest that cytokinin is important for bud-break. The position of the node on the Culm appears to affect bud-break and multiplication, middle nodes are the most suitable. Also, removal of prophylls enhances bud-break. High frequency multiple shoot induction was achieved from branch nodal segments collected from elite genotype on Murashige and Skoog’s (MS) medium supplemented with 1.5 mg/l 6-Benzylaminopurine (BAP) and 2.0 mg/l Kinetin (Kn). The size of explants and season greatly influenced the frequency of bud break. Best rooting response was observed on of Indole- 3 Butyric acid (IBA) 2mg/l with 60 % rooting. In vitro raised plants were successfully acclimatized and established in the field conditions where they exhibited normal growth.

Keywords: MS media, Bambusa, Tissue culture and Multiple shoot
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How to cite this article:

Hardik Gajjar, Akhil Raval, Hemangini Raval, Harikrushna Patel and Suthar Ramchandra. 2017. In Vitro Propagation of Bambusa vulgaris Through Branch Node.Int.J.Curr.Res.Aca.Rev. 5(8): 12-17
doi: https://doi.org/10.20546/ijcrar.2017.508.002