Abstract            Volume:4  Issue-9  Year-2016         Original Research Articles

Online ISSN : 2347 - 3215 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcret@gmail.com

A defined nutrient medium consists of inorganic salts, a carbon source, vitamins, and growth regulators. Other components may be added for specific purposes. They include organic nitrogen compounds, organic acids, and plants extracts. The Murashige and Skoog (MS) or Linsmaier and Skoog (LS) are the most widely used salt compositions, especially in procedures where plant regeneration is the objective. The B5, Nitsch and Nitsch (NN) media is also having importance in tissue culture. Between the two different media such as MS and B5 with 3mg/l BAP + 0.05 mg NAA tested for Morus Spp. varities M₅, S₃₆, V_I and Anantha, MS medium was found to be best basal medium for shoot induction when compared to the B5 media.    Further, the auxiliary buds exhibited high percentage of callus formation and weight of callus in all the mulberry varieties. It concluded that MS media is suitable for mulberry invitro regeneration.

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