Abstract            Volume:14  Issue-4  Year-2026         Original Research Articles

Online ISSN : 2347 - 3215 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcret@gmail.com

Effluent treatment through biological methods have gained attention, in particular to remove heavy metals and toxic intermediates from pharmaceutical industry was focused in this study. Using Spirulina platensis pellets the treated and untreated effluent were compared based on AAS analysis. In this study, Heavy metal removal study was further conducted for Lead. For the same Lead acetate (1g/100ml) incorporated with Zarrocks medium and applied for immobilized S.platensis cultivation. After incubation for 7 days the filtrate was collected and analyzed through Atomic Absorption Spectroscopy. The results showed lead concentration before treatment as 4.077mg/l and after treatment 1.719mg/l. Further SEM analysis was performed analysis revealed noticeable morphological changes on the surface of Spirulina platensis after exposure to heavy metals. The untreated cells exhibited a smooth, intact surface, while the treated samples showed irregularities, surface roughness, and the presence of particulate deposits, likely corresponding to absorbed heavy metal ions. Based on indexing method analysis, this study concluded that lead and chromium were the most effectively removed heavy metals, as confirmed by the Heavy metals Index (HMI) for Spirulina platensis immobilized pellets.

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