Abstract            Volume:13  Issue-5  Year-2025         Original Research Articles

Online ISSN : 2347 - 3215 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcret@gmail.com

Sperm DNA fragmentation (SDF) has emerged as a vital biomarker in the evaluation of male fertility, providing insights into sperm genetic integrity beyond standard semen parameters. Traditionally, SDF is assessed using manual laboratory techniques such as the TUNEL assay, SCSA (Sperm Chromatin Structure Assay), or the Comet assay, which require skilled personnel and are subject to procedural variability. Among these we have used SCSA (Sperm Chromatin Structure Assay). With the advent of automated systems like PRIME SPERM CASA, there is a growing interest in integrating digital technology into DNA fragmentation assessment for enhanced precision and consistency. This study compares the results of manual SDF assessments with those obtained through the PRIME SPERM CASA system. Findings indicate that while the automated system offers improved reproducibility and reduced operator dependency, it may differ in sensitivity or specificity depending on assay calibration and imaging parameters. The study underscores the importance of standardizing protocols and interpreting automated results within a clinical context to maximize diagnostic value and guide fertility treatment strategies.

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