Abstract            Volume:11  Issue-12  Year-2023         Original Research Articles

Online ISSN : 2347 - 3215 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcret@gmail.com

The cytotoxicity effect concentration of packaged food products was assessed by using Vero cell line were measured. Sample of packaged food products were collected from the shops located at Mogappair East (Chennai). To analyze the cytotoxicity, the cells were treated with various concentration (1000, 500, 250, 125, 62.5, 31.2, 15.6 and 7.8µg/ml) of 20 different samples, the cell viability was determined by the MTT assay. The average concentration of packaged food samples was selected was evaluate by Spectrophotometer. Cytotoxicity assay of food sample against normal cell line was conducted using Vero cell line. The percentage of nhibition was calculated and expressd as IC50. IC50 values below this stringent point were noted with twenty samples at six of the twenty studied should cytotoxicity effect. Lower doses enhanced the viability of the Vero cells by (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) MTT assay .higher doses decreased viability of the cells by 50% or more. MTT assay was used to measure the cell viability and survival. Amongst fourteen samples, Sample-1, Sample-2, Sample-3, Sample-4, Sample-5 and Sample-6 showed less than 50% cell viability in Vero cell lines and were studied for further cytotoxic effects. The study revealed that this is a first attempt in testing cytotoxicity in packaged food products and we showed successfully the cytotoxicity effect in six packaged food products out of twenty.

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