Abstract            Volume:11  Issue-5  Year-2023         Original Research Articles

Online ISSN : 2347 - 3215 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcret@gmail.com

Phosphate solubilise bacteria (PSB) has the ability to convert insoluble phosphates in to soluble phosphates. Characterization of this bacterium as inoculants in acidic soils increases its phosphorus uptake by the respective plant as well as its productivity. This research was conducted on low pH soils (<6.00) of Tsegede area, located in Western zone of Tigray region. The study revealed that a series of morphological and biochemical test were conducted, all the isolated phosphate solubilize bacteria were indicated gram negative, rod shaped and mucous producing. All of the strains were grew well at pH 6 and 7, temperature 15-35 ^oC and at 4% salt concentration. A positive response of the tested isolates was shown for 1.5 mg/l and 3.0 mg/l Mn SO₄ and 0.25 mg/l Zn SO_4. The anticipated significant response of antibiotic resistance was recorded. Hundred percent of the isolates were utilize L-arginine as N source as well as D-glucose, D-fructose and Lactose as of carbon sources. The highest solubilization index (SI) was observed from isolate ESB (2.21) followed by ESE (2.18) and the smallest SI was recorded from CHB, ITA and ESC (1.67). The highest colony diameter was obtained from Chegargudo at soil pH of 5.30 and from Endaslasea at soil pH of 4.92. The lowest colony diameter (4.00mm) was found at soil pH of 5.70 and 5.76. The tested phosphate solubilize bacteria had also a significant response after they grow on PVC broth media, accordingly the highest PS was obtained from FSA (30.70 ESB mg L^-1) followed by ITA (29.96 mg L^-1). The potential of the collected PSB to release phosphate indicates that a drop down of pH values from7.0 to 4.9.

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